| Volume 26 Issue 11-12 - Publication Date: 1 November- December June 2007 |
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| Injection Guidance System for Cellular Microinjections |
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| P. Kallio, T. Ritala, M. Lukkari, and S. Kuikka Institute of Automation and Control,
Tampere University of Technology,
PL 692, 33101 Tampere, Finland |
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| In this paper we present a novel injection guidance system that assists
an operator in the microinjection of living adherent cells. The system
includes a micromanipulator, an injection system, an impedance measurement
device and control software. The impedance measurement
system detects changes in the resistance of an injection capillary during
cell injections. From the measurement signal, the software determines
parameters that can be used to detect several injection events
such as cell–capillary contact, capillary breakage and capillary clogging,
which are all unavailable in the current microinjection systems.
Real cell injection experiments indicate that injection success rates
can be significantly increased: from 30–40% without the system to
65% with the system. |
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| Multimedia Key |
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Example
1: Illustration of the injection of
fluorescein isothiocyanate into
MCF-7 cells. (30 MB) avi |
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